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Application for windows and mac using python

I'm in a dilemma. I've got a python code that works for each of the yellow squares shown below but I want to make an application that looks like below and uses the information from the first text box and the second drag and drop box. Then depending on what the user clicked on, the code for that would run. I'm not sure how to approach this. Any help would be greatly appreciated!

You can use Python GUI libraries like:
Tkinter
PyQT
WxPython
Kivy
Pyglet
(This list is not exhaustive.)
Each has their own advantages and disadvantages. Choose the one that fits your project the best.
My personal recommendation for your particular project would be Kivy.

Embedding Chrome browser in python QT GUI?

I have a python GUI that opens a Chrome window using Seleium. Is there any way in the PyQT GUI to embed the Chrome browser's window so there are not 2 separate windows and its just the GUI? Guessing not possible but worth it to ask.

I have been looking to do exactly the same. I don't have a complete answer but hopefully some of what I found will be of use to others who are trying to do this as well.
It IS possible to embed the window of an outside application into a Python QT application. While I could quote various reference pages I have found that the following question gives most of the information and a good starting point to find more:
QT 5.5 embed external application into QWidget
Now, that requires knowing the Window ID but that isn't too hard to find. You can do that in Windows at least as described here:
http://timgolden.me.uk/python/win32_how_do_i/find-the-window-for-my-subprocess.html
As I said, this isn't a complete answer but should put anyone else following this trail several steps closer to the complete answer. Good luck everyone and if you find the complete answer please share it.

making a python gui for ffdshow

I was thinking that for a learning project for myself, I would try to make a GUI for ffdshow on linux, using tkinter. Wanted to make sure this project would be feasible first, before I get halfway through and run into something that cant be done in python.
Basic idea is to have a single GUI window with a bunch of drop down boxes that have the various presets (like format or bitrate), as well as a text box where a custom number can be entered if applicable. Then when all the options are selected, the user hits the Start button on the GUI and it shows a progress little bar with a percentage. All the options selected would just send the relevant selections as cli arguments for ffdshow, and begin the conversion progress (essentially turning all the user's input into a single perfect cli command).
Is all this doable with python and tkinter? and is it something that a relative newb with only very basic tkinter experience could pull off with books and other python resources?
Thanks

That is precisely the type of thing that python and Tkinter excel at. And yes, a relative newbie can easily do a task like that.

In wxPython, What is the Standard Process of Making an Application Slightly More Complex Than a Wizard?

I am attempting to create my first OS-level GUI using wxPython. I have the book wxPython in Action and have looked at the code demos. I have no experience with event-driven programming (aside from some Javascript), sizers, and all of the typical GUI elements. The book is organized a little strangely and assumes I know far more about OS GUI programming than I actually do. I'm fairly recent to object-oriented programming, as well. I'm aware that I am clearly out of my depth.
My application, on the GUI side, is simple: mostly a set of reminder screens ("Turn on the scanner," "Turn on the printer," etc) and background actions in Python either in the filesystem or from hitting a web service, but it is just complex enough that the Wizard class does not quite seem to cover it. I have to change the names on the "Back" and "Next" buttons, disable them at times, and so forth.
What is the standard process for an application such as mine?
1) Create a single wxFrame, then put all of my wxPanels inside of it, hiding all but one, then performing a sequence of hides and shows as the "Next" button (or the current equivalent) are triggered?
2) Create multiple wxFrames, with one wxPanel in each, then switch between them?
3) Some non-obvious fashion of changing the names of the buttons in wxWizard and disabling them?
4) Something I have not anticipated in the three categories above.

I don't have a good understanding of your application, but trying to force wxWizard to suit your needs sounds like a bad idea.
I suggest checking out the Demos available from the wxPython website. Go through each demo and I bet you'll find one that suits your needs.
I've personally never used wxWizard as I find it too cumbersome. Instead, I create a sequence of dialogs that do what I need.

Something like Explorer's icon grid view in a Python GUI

I am making a Python gui project that needs to duplicate the look of a Windows gui environment (ie Explorer). I have my own custom icons to draw but they should be selectable by the same methods as usual; click, ctrl-click, drag box etc. Are any of the gui toolkits going to help with this or will I have to implement it all myself. If there aren't any tools to help with this advice would be greatly appreciated.
edit I am not trying to recreate explorer, that would be madness. I simply want to be able to take icons and lay them out in a scrollable window. Any number of them may be selected at once. It would be great if there was something that could select/deselect them in the same (appearing at least) way that Windows does. Then all I would need is a list of all the selected icons.

Python has extensions for accessing the Win32 API, but good luck trying to re-write explorer in that by yourself. Your best bet is to use a toolkit like Qt, but you'll still have to write the vast majority of the application from scratch.
Is there any way you can re-use explorer itself in your project?
Updated for edited question:
GTK+ has an icon grid widget that you could use. See a reference for PyGTK+: gtk.IconView

In wxPython there's a plethora of ready-made list and tree controls (CustomTreeCtrl, TreeListCtrl, and others), a mixture of which you can use to create a simple explorer in minutes. The wxPython demo even has a few relevant examples (see the demo of MVCTree).

I'll assume you're serious and suggest that you check out the many wonderful GUI libraries available for Python.