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How to click the same button on Tkinter python once the process completed with threading

I have simple program with only one function which process files and then converts to pdf on a press of button. initially GUI window freezes while the function was running, after this I introduced threading into the program and now GUI freezing issue was resolved, but now when press the button again it shows below error
if anyone can help me on how to fix this and also explain how it works, that will be really helpful, as I am new to python and just started using Tkinter.
sample code would be even more helpful.

You shouldn't use the old thread object you used on the first button click. Instantiate a new one instead.
This is because, you can't start the same thread twice.
So maybe keep a list of threads and add a new one with each button click.

How to implement "software interrupt"?

I am trying to create an "emergency stop" button that will immediately stop / break a linear code execution, but I am unable to come up with a solution. We have a GUI running in a browser and when a button is pushed an MQTT messages is received by the back end (which is written by me in Python).
There are different threads and the message is received, but when in another thread an if statement is running I am unable to figure out how to stop / break the linear code execution in that thread right away. Is there a good method for this?
It would be also OK if I could stop the entire thread and afterwards start it again (if another button is pushed). How could I do that?
I mean. I could probably solve this right away if I would be using a hardware switch and an interrupt (the code is running on a Raspberry Pi by the way). But I do not know how to "simulate" an interrupt and an interrupt service routing in a software. I most definitely can't be the only person facing the same problem.

Stuck in a loop - Threaded continues while loop prevents code from moving forward (Python)

EDIT:
I apparantly wasn't aware of daemon threads.. This solved the main issue..
Now just puzzling to keep the loop checking for button press, whether it has been pressed or not =)
I'm trying to stop a raspberry pi running code when I press a button (emergency stop button connected to the pins).
So far it's not working out. (In my example code I use code to switch the light of the button to make testing easier).
The threading seems to be working well, however the shell program installed in the pi running the code, first 'test runs' the code to check for errors before actually executing. In my while not read_button() code, the 'checking' of the code gets stuck on the 'while not' until I press the button; not ideal..
Reversing the code and having while read_button() or only if read_button() doesn't actually respond to my button press, since likely the code finishes after checking instead of continuously check.
So.. How to solve this? A continues loop stops my code from proceeding until the while loop is broken (I did join the thread in the end..). A non-continues loop doesn't function, since it won't continuously check for the button press..
To make things worse; after the emergency stop was performed I want the thread to still continue checking for button press after (the user might decide to resume the program and have to hit emergency stop again later on the way within the same code).
I feel like I'm in an endless loop myself trying to fix this =P
(Sorry for the lengthy post and sorry for any noob-terms used, I'm very new to this; only picked programming up 3 weeks ago)
Thank you very much in advance!!
def button_check():
while not read_button(): #developer API
if read_button():
set_button_light(blue=True) #developer API
t = Thread(target=button_check)
t.start()
time.sleep(20) #just to give me time to test
t.join()

Python GUI using os.system to run python script cause main GUI "Not Responding"

StackOverflow community.
I am writing a python GUI to monitor another program's data in OSX environment and at one point I decide to click one button to open another python script that I wrote. It does work but it also causes a lag problem of the main GUI program as soon as I click the button. For the lag problem I mean the GUI window is "Not Responding" and I need to force quit. The method I use to run the new script is,
def create_html():
os.system('python realtime.py')
My program doesn't have the multiple class structure, just the simple canvas, and framework. I wonder if this is also the problem to cause my program running slow.

The problem is you are using os.system, which is a blocking call. It will not return control to your main code until python realtime.py is done executing and returned.
You need to use a call that will not block the rest of your program, such as subprocess.Popen.
You can also see this QA for further information

Tkinter threading freezes after executing twice

I have recently started using multithreading to be able to, for example, supply input during a for loop. This was successfull but I wanted to try more.
When I tried threading in combination with Tkinter things went wrong.
So for my example I have a very simple piece code (Python 2.7.11). I realize it beats the point of threading but it shows my problem:
import Tkinter
import threading
def func():
root=Tkinter.Tk() #Open Tkinter window
Tkinter.mainloop()
root.quit() #Extra quit, although it doesn't seem to help
thread1=threading.Thread(target=func)
thread1.start()
thread1.join() #Wait for thread to end (for me to close the window)
del thread1 #Extra deletes (although they don't seem to help)
del func
Running this code once works fine. The Tkinter window opens, I close it manually and the rest works fine. However if I run it again the interpreter freezes. If a second thread, or a simple loose print statement, is added it doesn't even start those. The entire enterpreter freezes and I can only re-run this code by restarting the Kernel.
So do I have to quit the thread/Tkinter in a special way to prevent this from happening?
Additional information:
My CPU usage goes way up. When running it the 2nd time task manager shows up to 30% CPU usage. So I guess there is an infinite loop going on somewhere.
I have read that Tkinter does not take threading well, but I have seen instances where this workes. What I have here is a very simple thing so it must be something I am doing wrong.
Hopefully someone can help me, because I couldn't find an answer in whatever way I tried to Google it.
Thanks in advance.